Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme.
نویسندگان
چکیده
A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.
منابع مشابه
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ورودعنوان ژورنال:
- Chemical communications
دوره 18 شماره
صفحات -
تاریخ انتشار 2009